RESEARCH ASSOCIATES AND PROJECTS:

Mansoureh Eghbali Ph.D. , Research Associate

Mechanisms of hormonal control of ion channels in myometrium.  Molecular mechanism and metabolic pathways of K+ channel remodeling in heart functional hypertrophy during pregnancy. 

Tamara Minosyan, B.Sc., Laboratory Assistant

Heart ion channel targeting to lipid rafts. Regulatory molecular complexes and scaffolding proteins of heart K+ channels.

Tomoaki Saito, Ph.D. Postdoctoral Fellow

 

Electrical heart remodeling under the influence of sex hormones and during gestation.

 

Yi-Kuang Liu, Ph.D. Postdoctoral Fellow

 

Optical techniques beyond the diffraction limit to start defining the molecular identity of proteins in the cardiovascular system.

 

Andrea Ciobotaru, Staff  Research Associate (PreMed Student)

 

Heart estrogen receptor regulated genes.

 

Patricia Fishader (MD, Clinical Trainee)

 

Signaling cascade of heart estrogen receptor.

 

Techniques:

General electrophysiology: Ionic and gating currents, noise analysis and single channel measurements in expressed channels.  Patch recordings in heart and smooth muscle cells.  Mechanical recordings in heart and smooth muscle cells.

Optical Techniques: Development of microscopy beyond the diffraction limit to start defining the molecular identity of proteins in the cardiovascular system. High resolution confocal microscopy with 3 D reconstruction for protein localization in cellular compartments. Identification of molecular partners with proteomics.

Molecular biology: RT-PCR, Real Time PCR, RNAse protection assay, microarray technology, mutagenesis, molecular cloning, cell transfection, etc.

Cell Biology: Western blot analysis, subcellular centrifugation, immunocytochemistry, protein colocalization, trafficking, FRET, Ca2+ transients, etc. 

Small Animal Physiology: Assesment of cardiovascular function with non-invasive and imaging techniques.

E-mail to: estefani@ucla.edu

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