MANSOUREH EGHBALI, Ph.D.

 

                Assistant Professor

 

 

 

 

Division of Molecular Medicine

Department of Anesthesiology

David Geffen School of Medicine at UCLA

Los Angeles, California.

Los Angeles, California 90095-7115, USA

 

 

Phone: (310) 794 7808

Fax: (310) 825 6649

Email: meghbali@ucla.edu

 

 

 

ACADEMIC POSITIONS

2001-2007:         Assistant Researcher, Department of Anesthesiology, UCLA, Los Angeles, California.

2007-present     Assistant Professor, Department of Anesthesiology, UCLA, Los Angeles, California.

Education:

1983 - 1987        Bachelor of Biomedical Engineering, University of Kerman, Iran

 

1993 - 1995        Master of Biomedical Engineering, University of New South Wales, Sydney, Australia

 

1996 – 1999       Ph.D, John Curtin School of Medical Research, Australian National University, Canberra, Australia

 

1999 – 2001       Postdoctoral Fellow, Department of Anesthesiology, UCLA, Los Angeles, California.

 

 
 
Grant Support

 

Active

 

7/2004-6/2008             American Heart Association, Scientist Development Grant “Heart MiRP1 gene regulation: Gender and Age Differences”. (PI).

4/2007-3/2012             NIH BRG R01HL088640. “Revealing Cardiovascular Stress Regulation beyond the Diffraction Limit”. (Co-Investigator).

 

Pending

 7/2007-6/2012             NIH R01HL089876 “Cardiac KCNE2 (MiRP1) Regulation by Estrogen and Cardiac Stress”. (PI).

 

Completed

 7/2002-6/2004             American Heart Association, Postdoctoral Fellowship. “Regulation of ITO current molecular components.”

 

Research Statement

 

My main interest is to unravel: 1. The molecular basis of the remodeling of voltage activated K channels in heart during cell function and disease and 2. The cardio-protective mechanism of hormones. I am investigating the role of sex hormones in the remodeling of cardiac KCNE2 (MiRP1) regulatory beta subunits and the molecular events (gene activity, protein expression and signaling cascades) triggering the installation and recovery of functional heart hypertrophy during pregnancy.

I am using a multidisciplinary approach including molecular biology, biochemistry, electrophysiology and proteomic techniques in conjunction with high resolution confocal microscopy and protein-protein proximity analysis.I have established collaboration efforts with faculty members of the Division of Molecular Medicine and the Department of Physiology. I have a history of continuous AHA funding from 2002 (AHA Postdoctoral Fellowship 7/2002-6/2004 and AHA SDG 7/2004-6/2008). I have submitted my first NIH RO1 and I am co-investigator in the NIH BRG R01HL088640 (PI Dr. Stefani). I am already planning my 2nd RO1 as a standing along grant or as part of a PPG.I am totally committed to excellence in science with the conviction that I will continue to grow in the exciting environment of the cardiovascular community at UCLA and that I will contribute to the UCLA research enterprise.

 1. Cardiac KCNE2 (MIRP1) Regulation by Estrogen and Cardiac Stress

KCNE2 (MiRP1) beta subunit assembles with ERG1 channels regulating their activity. Mutations in the KCNE2 subunit are associated with arrhythmogenesis and long QT syndrome. I found that mouse cardiac KCNE2 transcript and protein levels are upregulated in females in conditions of high estrogen. Our main hypothesis is that estrogen is a central physiological mechanism that positively regulates cardiac KCNE2 transcript (genomic mechanism), protein levels and direct its subcellular targeting to the T-tubular membranes. We are investigating i) the role of estrogen and cardiac stress on the expression, localization and function of cardiac KCNE2, ii). Whether a genomic action of estrogen is a mechanism contributing to the upregulation of KCNE2 expression, iii) Possible changes in KCNE2 complex formation explaining differential targeting or function under the influence of estrogen or heart stress. These studies will provide new information on KCNE2 molecular partners and the role of KCNE2 in determining cardiac excitability in health and in failing hearts.

This program is the basis of the active ·AHA Scientist Development Grant and the submitted NIH RO1 grant.

 2. Molecular events involved in the installation and recovery of functional heart hypertrophy during pregnancy

During pregnancy, there is an increased risk of arrhythmias and the heart develops functional hypertrophy. I am proposing that, in addition to mechanical stress, the rise of estrogen toward the end of pregnancy contributes to pregnancy-related heart hypertrophy by increased c-Src activity and that the rise of estrogen is one factor that down regulates cardiac Kv4.3 gene expression providing a molecular correlate for a longer QT interval in pregnancy. My main goal is to unravel the cellular/molecular mechanisms, which are used to make the pregnant heart work more efficiently and recover to normal cardiac function postpartum. Our current knowledge points to the view that Kv4.3, c-Src, estrogen receptors (possibly estrogen receptor alpha) are key signaling molecules in pregnancy-related heart hypertrophy but upstream, downstream or parallel signals are still unknown for this natural form of hypertrophy. Clues for protective mechanisms may be derived from the rapid recovery that occurs postpartum.

This program is the basis for my second NIH RO1 grant planned for February 2008.


 

Publications

 

1. Eghbali M., Curmi J.P., Birnir B., & Gage P.W. (1997). Hippocampal GABAA channel conductance increased by diazepam. Nature 388, 71-75.

2. Eghbali M., Gage P.W. & Birnir B. (2000). Pentobarbital modulates g-aminobutyric acid-activated single-channel conductance in rat cultured hippocampal neurons. Mol. Pharmacol 58(3):463-469.

3. Birnir B., Eghbali M., Everitt A.B. & Gage P.W. (2000). Bicuculline, pentobarbital and diazepam modulate spontaneous GABAA channels in rat hippocampal neurons, British Journal of Pharmacology 131: 695-704.

4. Birnir B., Eghbali M., Cox G.B. & Gage P.W. (2001). GABA concentration sets the conductance of delayed GABAA channels in outside-out patches from rat hippocampal neurons. Journal of membrane biology 181(3):171-183.

5. Zarei M.M., Zhu N., Alioua A., Eghbali M., Stefani E. & Toro L (2001). A novel MaxiK splice varient exhibits dominant negative properties. Journal of Biological Chemistry 276(19):16232-16239.

6. Song M., Helguera G., Eghbali M., Zhu N., Zarei M. M., Olcese R., Toro L. & Stefani E (2001). Remodeling of kv4.3 potassium channel gene expression under the control of sex hormones. Journal of Biological Chemistry 276(34):31883-90. In this paper Song, Helguera and Eghbali equally participated.

7. Eghbali M., Olcese R., Zarei M.M., Toro L. & Stefani E. (2002). External pore collapse as an inactivation mechanism for kv4.3 k+ channels. Journal of Membrane Biology 188(1):73-86.

8. Eghbali M., Gage P.W. & Birnir B. (2003). Effects of propofol on GABA(A) channel conductance in rat-cultured hippocampal neurons. Eur J Pharmacol. 468(2):75-82.

9. Eghbali M., Birnir B & Gage P.W. (2003). Conductance of GABAA channels activated by pentobarbitone in hippocampal neurons from newborn rats. Journal of Physiology. 552(1):13-22.

10. Eghbali M., Toro L. & Stefani E. (2003). Diminished surface clustering and increased perinuclear accumulation of large conductance Ca2+-activated K+ channel in mouse myometrium with pregnancy. Journal of Biological Chemistry 278(46): 45311-7.

11. Nishimaru K., Eghbali M., Stefani E. & Toro L.(2004). Function and clustered expression of MaxiK channels in cerebral myocytes remain intact with aging. Exp Gerontol. 39(5):831-839.

12. Zarei M.M., Eghbali M., Alioua A., Song M., Knaus H.G., Stefani E., Toro L. (2004). An endoplasmic reticulum trafficking signal prevents surface expression of a voltage- and Ca2+-activated K+ channel splice variant. Proc Natl Acad Sci U S A. 101(27):10072-10076. In this paper Zarei, Eghbali and Alioua equally participated.

13. Nishimaru K., Eghbali M., Lu R., Marijic J., Stefani E. & Toro L. (2004). Functional and molecular evidence of MaxiK channel β1subunit decrease with coronary artery ageing in the rat. Journal of Physiology 559(3): 849–862.

14. Stefani E, Eghbali M., Minosyan TY, Alioua A & Toro L,. (2004). Molecular studies in heart hypertrophy during pregnancy. J. Muscle Res Cell Motil. 25(8): 607.

15. Eghbali M., Deva R, Alioua A, Minosyan TY, Ruan H, Wang Y, Toro L, Stefani E. (2005). Molecular and functional signature of heart hypertrophy during pregnancy. Circulation Research. 96(11):1208-16.

16. Zhu N, Eghbali M., Helguera G, Song M, Stefani E, Toro L. (2005). Alternative splicing of Slo channel gene programmed by estrogen, progesterone and pregnancy. FEBS Letter. 579(21): 4856-60.

17. Lu R. , Alioua A. , Kumar Y., Eghbali M., Stefani E.,&Toro L. (2006). MaxiK     channel partners: physiological impact. J Physiol 570.1: 65–72.

18. Tanaka Y., Tang G., Takizawa K., Otsuka K., Eghbali M., Song M., Nishimaru K., Shigenobu K., Koike K., Stefani E. & Toro L (2006). Kv channels contribute to NO- and ANP-induced relaxation of a rat conduit artery. J Pharmacol Exp Ther. 317(1):341-54.

19. Eghbali M., Wang Y., Toro L. & Stefani E. (2006). Heart Hypertrophy during pregnancy: a better functioning heart? Trends in cardiovascular Medicine. 16(8):285-291.

20. Ropero A.B., Eghbali M., Toro L.& Stefani E. (2006). Heart Estrogen Receptor Alpha: Distinct Membrane and Nuclear Distribution Patterns and Regulation by Estrogen. J. Mol. Cell Cardiol. 41(3):496-510.

21. Cavalli A., Eghbali M., Minosyan T.Y., Stefani E.& Philipson K. (2007). Localization of sarcolemmal proteins to lipid rafts in myocardium. Cell calcium. doi:10.1016/j.ceca.2007.01.003. In this work Cavalli and Eghbali contributed equally.

22. Minosyan T., Lu R., Eghbali M., Toro L. & Stefani E. (2007). Increased 5-HT contractile response in late pregnant rat myometrium associates with a higher density of 5-HT2A receptors. J Physiol. 581(Pt 1):91-7.

23. Alioua, A., Lu R., Kumar Y., Eghbali, M., Toro L. & Stefani, E. Slo1 caveolin-1 binding motif, a mechanism of caveolin-1-slo1 interaction regulating slo1 expression. Under review (Journal of Biological Chemistry).

24. Lu R., Alioua A., Kumar Y., Eghbali M., Stefani E. and Toro L. 5-HT2A receptor –c-Src signaling is supported by their close association and is upstream of RhoGTPase kinase. Under Review (Journal of Physiology).

25. Kundu P., Ciobotaru A., Foroughi S., Toro L. and Eghbali M. Estrogen stimulation of KCNE2 (MiRP1) promoter activity by a direct genomic mechanism. Submitted.

26. Saito T., Ciobotaru A., Toro L., Stefani E. & Eghbali M. Gender and Estrogen Determines Outward Potassium Current Densities in Mouse Ventricular Myocytes. Submitted.

 

Finalized to be Submitted

 

26. Helguera H., Eghbali M., Sforza D., Toro L. & Stefani E. Changes in global gene expression in rat myometrium in the transition from late pregnancy to parturition.

27. Vallejo J., Eghbali M., Toro L. & Stefani E. Estrogen induced accumulation of large conductance Ca2+-activated k+ channels into the sarcoplasmic reticulum of mouse myometrium.

 

 
In Preparation

29. Eghbali M., Minosyan T., Toro L. & Stefani E. Kv4.3 and c-Src Can Share the Same Membrane Microenvironment with Caveolin in Rat Heart.

30. Eghbali M., Toro L. & Stefani E. High External K+ Promotes Faster Inactivation in Kv4.3 Channels and Heart ITO Currents.

31. Toro L., Conti L., Eghbali M., Mahajan A., Tanaka Y., Stefani E., Vandenberg C. &. Alioua A. Inward rectifier K+ channels (Kir) control muscle tone of a rat conduit vessel: Role of Kir2.x.

32. Marijic J., Lee J.Y., Eghbali M., Song M. Stefani E. & Toro L. Aging alters regional vascular responses to vasopressin.

 

Book Chapters

L.Toro, A. Alioua, R. Lu, J. Garcia-Valdes, M. Zarei, K. Nishimaru, M. Eghbali, and E. Stefani (2003). Ca2+-activated, voltage-dependent K+ channels. Ion Channels in the Pulmonary Vasculature. Lung Biology in Health and Disease. C. Lenfant and J. X.-J. Yuan, eds. Marcel Dekker, Inc. Vol. 197 :237-256.

 

Gene Bank Submission

 

1. Eghbali M., Zhu N., Toro L. & Stefani E. (2001). Rattus norvegicus transient voltage dependent potassium channel Kv4.3 long form mRNA, complete cds. Gene Bank, Accession Number AF334791.

2. Garcia-Valdes, J., Eghbali, M., Stefani, E. & Toro, L (2001). Mouse Kcnmb2 subunit of the large conductance calsium activated K+ channel. Complete cds. Gene bank, Accession Number AY062429.

3. Eghbali M., Foroughi S., Toro L. and Stefani E. (2003). Rattus norvegicus inactivating beta 2 subunit of large conductance Ca2+-activated K+ channel mRNA, complete cds. Gene bank, Accession Number AY191836.

 

Abstracts

1. Eghbali M., Birnir B., Curmi J.P. & Gage P.W. (1997). Diazepam increases the conductance of hippocampal GABAA channels. Proceedings of A.P.P.S. 28(2), 50.

2. Birnir B., Eghbali M., Curmi J.P., Cox G.B. & Gage P.W. (1998). Modulation of GABAA single channel conductance. Biophysical Journal, M-P327.

3. Eghbali M., Birnir B. & Gage P.W. (1998). Direct activation of GABAA receptors by pentobarbitone in cultured rat hippocampal neurons. Proceedings of A.P.P.S. 29(2), 119p.

4. Birnir B., Eghbali M., Cox G.B. & Gage P.W. (1998). GABAA single channel conductance is determined by the GABA concentration in outside-out patches from cultured rat hippocampal neurons. Proceedings of A.P.P.S. 29(2), 315p.

5. Birnir B., Eghbali M., Everitt A.B., Cox G.B. & Gage P.W. (1999). Non-synaptic GABA-gated receptors exhibit great plasticity in channel conductance. Biophysical Journal, Tu-P357.

6. Birnir B, Eghbali M & Gage P.W. (1999). Channel conductance of GABAA receptors varies with agonist and modulator concentration. Proceedings of A.P.P.S. 30(2), 65P.

7. Song M., Zhu N., Eghbali M., Toro L. & Stefani E. (2000). Changes of expression levels of Kv4.3 K channels during pregnancy, Biophysical Journal, W-P2648.

8. Eghbali M., Olcese R., Zarei M.M., Toro L. & Stefani E. (2001). External k+ increases the rate of inactivation of kv4.3 k+ channels, Biophysical Journal, 1888-P.

9. Zarei M.M., Zhu N., Alioua A., Eghbali M., Stefani E. & Toro L. (2001). A novel MaxiK splice variant exhibits dominant negative properties. Biophysical Journal, 887-P.

10. Stefani E., Song M., Eghbali M., Olcese R., Helguera G. & Toro L. (2001). -estradiol induces a specific down regulation of Kv4.3 potassium channel protein expression in rat myometrium. The Endocrine Society Meeting. Denver, CO.June 20-23.

11. Birnir B., Eghbali M. & Gage P.W. (2001). The agonist sets the conductance level of GABAA channels. Society for Neuroscience, 460.5 page 237.

12. Stefani E., Song M., Zhu N., Helguera G., Olcese R., Eghbali M., & Toro L. (2001). Hormonal regulation of ion channels. Workshop on genomics vs non-genomic steroid actions. Spain 131-P31.

13. Eghbali M., Olcese R., Zarei M.M., Toro L. & Stefani E. (2002) . External pore collapse as an inactivation mechanism for Kv4.3 K+ channels. Biophysical Journal, 1117-P.

14. Nishimaru K., Eghbali M., Stefani E. & Toro L. (2002). Aging-associated changes in Maxik channel properties in rat coronary artery. Biophysical Journal, 1198-P.

15. Helguera G., Sforza D., Eghbali M., Toro L & Stefani E. (2002). Changes in global gene expression in rat myometrium at the onset of labor. Biophysical Journal, 3056-P.

16. Eghbali M., Song M., Zarei M.M., Helguera G., Stefani E., & Toro L. (2002). Different mechanisms (transcription vs. trafficking) of MaxiK channel surface expression in rat and mouse lead to diminished surface expression in late-pregnant myometrium. The Endocrine Society Meeting, P1-486.

17. Helguera G., Sforza D., Eghbali M., Toro L. & Stefani E. (2002). Changes in global gene expression in rat myometrium at the onset of labor. The Endocrine Society Meeting, P3-193.

18. Sforza D., Helguera G., Eghbali, M., Mahajan A. & Stefani E. (2002). Global Gene Expression in Mouse Ventricle during Pregnancy-Induced Cardiac Hypertrophy: Contribution of Cell Cycling and Cell Division Genes. American Society of Anesthesiology A-612.

19. Alioua A., Mahajan A., Eghbali M., Stefani E. & Toro L. (2002). Functional and Molecular Identification of Inward Rectifier Type 2 Family (Kir2.x) Channels in Rat Aorta Smooth Muscle. American Society of Anesthesiology A-631.

20. Nishimaru K., Eghbali M., Stefani E. & Toro L. (2002). Mechanism of MaxiK Channel Loss during Aging in Coronary Arteries: Transcriptional Downregulation of Both Pore Forming and Regulatory Subunits. American Society of Anesthesiology A-703.

21. Zhu N., Eghbali, M., Helguera G., Stefani E. & Toro L. (2002). -Estradiol Mediates Transcriptional Downregulation of a MaxiK Splice Variant Confering Differential PKA Dependent Phosphorylation. American Society of Anesthesiology A-707.

22. Eghbali, M., Song M., Helguera G., Toro L. & Stefani E. (2002). Different Cellular Mechanisms in Rats and Mice for the Reduction of BK(Ca++) Channel Surface Expression in Myometrium during Late Pregnancy. American Society of Anesthesiology A-1058.

23. Helguera G., Sforza D., Eghbali M., Toro L. & Stefani E. (2002). Global Gene Expression Changes of Rat Myometrium before and during Labor. American Society of Anesthesiology A-1059.

24. Eghbali M., Toro L. & Stefani E. (2003). High External K+ Promotes Faster Inactivation in Kv4.3 Channels and Heart ITO Currents. Biophysical Journal, 371-P.

25. Rueda A. Alioua A., Eghbali M., Song M., Stefani E. &Toro L. (2003). Expression and intracellular localization of sorcin in vascular smooth muscle. Biophysical Journal, 496-P.

26. Zarei M.M., Eghbali M., Song M., Stefani E. & Toro L. (2003). A Novel ER Trafficking Signal Prevents Surface Expression of a MaxiK Splice Variant. Biophysical Journal, 733 Platform.

27. Eghbali M., Minosyan T., Bravo-Zehnder M., Toro L. & Stefani E. (2003). Kv4.3 and c-Src Can Share the Same Membrane Microenvironment with Caveolin in Rat Heart. Biophysical Journal, 738 Platform.

28. Alioua A., Conti L., Eghbali M., Mahajan A., Tanaka Y., Stefani E., Vandenberg C. & Toro L (2003). Inward rectifier K+ channels (Kir) control muscle tone of a rat conduit vessel: Role of Kir2.x. Biophysical Journal, 1094-P.

29. Eghbali M, Alioua A., Toro L. & Stefani E. (2003). Increased c-Src Activity and Transcriptional Downregulation of Kv4.3 and KChIP2 in Heart Functional Hypertrophy During Pregnancy. Biophysical Journal, 2003-P.

30. Marijic J., Lee J.Y., Eghbali M., Song M. & Toro L.(2003). Aging alters regional vascular responses to vasopressin. American Society of Anesthesiology.A-655

31. Eghbali M., Minosyan T., Toro L. & Stefani E. (2003). Kv4.3 Channel, c-Src Tyrosine Kinase and Caveolin Form a Macromolecular Signaling Complex in Rat Heart. American Society of Anesthesiology.A-702

32. Eghbali M., Toro L. & Stefani E. (2003). High External K+ Modulates Activation and Inactivation of Kv4.3 K+ Channels and Heart ITO Currents. American Society of Anesthesiology.A-727

33. Lu R., Eghbali M., Song M., Stefani E. & Toro L. (2003). Coupling of c-Src to MaxiK Channels Mediating Vasoconstriction is Weakened by Age. American Society of Anesthesiology.A-745.

34. Alioua A. , Kumar Y., Eghbali M., Minosyan T. & Toro L. (2003). Maxi-K, c-Src and Caveolin form a Macromolecular Complex and Colocalize in Brain Lipid Rafts. American Society of Anesthesiology.A-800

35. Deva R. , Eghbali M., Toro L. and Stefani E. (2003). Transcriptional Regulation by Estrogen of Myometrium and Cardiac Kv4.3 K+ Channels. American Society of Anesthesiology.A-1167.

36. Minosyan T., Eghbali M., Alioua A., Lu R., & Stefani E. (2003). Macromolecular Signaling Complexes of Myometrium K+ Channels (MaxiK and Kv4.3). American Society of Anesthesiology.A-1219.

37. Eghbali M., Alioua A., Toro L. & Stefani E. (2003). Molecular Signature of Heart Functional Hypertrophy during Pregnancy. American Society of Anesthesiology.A-1560.

38. Zarei M.M. , Eghbali M., Song M., Stefani E. & Toro L. (2003). A new ER Trafficking Signal Identified in a MaxiK Splice Variant Prevents Protein Expression ASCB,43rd Annual Meeting. 1273-P.

39. Alioua A. , Kumar Y., Lu R., Eghbali M., Stefani E. & Toro L. (2004). Arterial MaxiK, c-Src and 5-HT2A Receptors Strategically Colocalize in Lipid Rafts and Form a Macromolecular Complex for Efficient Arterial Tone Control. Biophysical Journal, 49-Platform.

40. Deva R., Eghbali M., Ropero A.B., Toro L. &Stefani E. (2004). Heart Transcript Levels of Estrogen Receptor Alpha but not Beta Are Under Hormonal Control. Biophysical Journal, 255-P.

41. Ropero A.B., Deva R., Eghbali M., Alioua A., Toro L. &Stefani E. (2004). Estrogen Receptor Alpha Localizes In Several Subcellular Compartments In Adult Rat Cardiomyocytes. Biophysical Journal, 256-P.

42. Alioua A., Lu R., Garcia J., Eghbali M., Stefani E. & Toro L. (2004). Diminished MaxiK Channel Expression Alters 5-HT-Induced Contraction in Aged Rat Aorta. Biophysical Journal, 640-P.

43. Kumar Y., Alioua A., Eghbali M., Minosyan T., Stefani E. & Toro L. (2004). Brain MaxiK Channel, c-Src and Caveolin-1 Macromolecular Complex: Lipid raft and Microvessel Localization. Biophysical Journal, 642-P.

44. Lu R., Alioua A., Eghbali M., Stefani E. & Toro L. (2004). Activation of c-Src Tyrosine Kinase as a Primary Step in 5-HT Receptor Mediated Vasoconstriction is Supported by Their Colocalization in Aortic Myocytes. Biophysical Journal, 1193-P.

45. Minosyan T., Eghbali M., Alioua A., Toro L. &Stefani E. (2004). Serotonin Receptors, Caveolin, and c-Src Form Part of the Same Macromolecular Complex in Rat Myometrium and Are Upregulated in Late Pregnancy. Biophysical Journal, 1194-P.

46. Garcia J., Alioua A., Eghbali M., Stefani E. & Toro L. (2004). Different Pattern of Expression and Subcellular Localization of c-Src, Yes, and Fyn in Rat Aortic Myocytes. Biophysical Journal, 2037-P.

47. Eghbali M., Toro L. & Stefani E. (2004). High Resolution Confocal Microscopy and 3D Deconvolution Reveals that Membrane and Cytosolic Proteins Can Be Organized in Distinct Cluster Patterns. Biophysical Journal, 2310-P.

48. Eghbali M., Toro L. & Stefani E. (2004). MaxiK Channel Localizes in Dense Clusters at the Surface of Non-pregnant Mouse Myometrial Cells and Becomes Undetectable in Late Pregnancy. Biophysical Journal, 2705-Platform.

49. Ropero A.B., Eghbali M., Deva R., Toro L. &Stefani E. (2004). A 43kDa form of estrogen receptor alpha (ER) associates with membrane structures in rat male hearts. FASEB meeting.(Steroid Hormone Receptors) P3-2.

50. Zarei M.M, Eghbali M., Alioua A., Stefani E., &Toro L. (2004). A new ER Trafficking Signal Identified in a Smooth Muscle MaxiK Splice Variant Prevents Channel Protein Expression. American Society of Anesthesiology. A-671

51. Alioua A, Garcia J., Eghbali M., Lu R., & Toro L. (2004). c-Src, Yes, and Fyn in Rat Aortic Myocytes: Functional Role, Quantification and Subcellular Localization. American Society of Anesthesiology.A-672

52. Lu R., Alioua A., Eghbali M., Stefani E. & Toro L. (2004). Src Tyrosine Kinase Activation is Critical for 5-Hydroxytriptamine Contractile Signaling in Aortic Myocytes. American Society of Anesthesiology.A-675

53. Alioua A. , Kumar Y., Lu R., Eghbali M., & Toro L. Caveolae Disruption Uncouples 5-HT2A Receptor Contractile Signaling Complex CoAssembled by MaxiK, c-Src and Caveolin-1. American Society of Anesthesiology. A-687.

54. Ropero A.B., Eghbali M., Deva R., Toro L. &Stefani E. (2004). A 43kDa Form of Estrogen Receptor alpha Localizes in Several Subcellular Membrane Compartments In Adult Rat Cardiomyocytes. American Society of Anesthesiology.A-702.

55. Deva R., Eghbali M., Ropero A.B., Toro L. &Stefani E. (2004). Hormonal Regulation of Heart Transcript Levels of Estrogen Receptor Alpha. American Society of Anesthesiology. A-734

56. Minosyan T., Eghbali M., Ph.D., Alioua A., Toro L., & Stefani E., (2004). Regulation During Pregnancy of the Macromolecular Complexes Formed by Serotonin Receptors (5HT2A), Caveolin, and Tyrosine kinase (c-Src). American Society of Anesthesiology.A-1182.

57. Eghbali M., Deva R., Alioua A., Wang Y., Toro L. & Stefani E. (2005). Molecular Signature of Heart Hypertrophy in Mice During Pregnancy. Biophysical Journal, 585-P

58. Ropero A.B., Eghbali M., Toro L. &Stefani E. (2005). Different Subcellular Localization but Similar Downregulation Regulation by Estrogen of the Two Estrogen Recetpor alpha Isoforms of 66 and 43 kDa in Adult Rat Cardiomyocytes. Biophysical Journal, 1512-P

59. Stefani E., Grabarchuk V., Eghbali M., Ropero A.B. &Toro L. (2005). Discrete Protein-Protein Proximity (“Colocalization”) Analysis in Microscopic Images. Biophysical Journal, 1674-P

60. Gomes P.B., Crosso C., Eghbali M., Ropero A.B., Alioua A., Toro L. &Stefani E. (2005). Upregulation of Kv4.3 channel activity by c-Src tyrosine kinase dependent phosphorylation. Biophysical Journal, 2235-P

61. Minosyan T., Eghbali M., Vallejo J., Toro L., Stefani E. (2005). Transcriptional and Translational Changes of 5-HT(2A) Receptors, c-Src, Caveolin 1 and 2, in Myometrium during Pregnancy in Rats. American Society of Anesthesiology. A-563

62. Vallejo J., Eghbali M., Toro L., Stefani E. (2005). Accumulation of Large Conductance Ca 2+ -Activated K + (MaxiK) Channels into the Sarcoplasmic Reticulum in Mouse Myometrium duri. American Society of Anesthesiology. A-565

63. Gomes P., Corsso C.D., Eghbali M., Toro L.& Stefani E. (2005). Modulation of Kv4.3 Currents by Src Tyrosine Kinase Phosphorylation. American Society of Anesthesiology.A-120

64. Lu R., Alioua A., Eghbali M., Stefani E., Toro L. (2005). 5-HT 2A Receptor-c-Src-Rho-Kinase Contractile Signaling Pathway in Aortic Myocytes American Society of Anesthesiology.A-519

65. Ropero A.B., Eghbali M., Kundu P., Toro L.& Stefani E. (2005). Membrane vs Non-Membrane Localization of Two Estrogen Receptor alpha Forms in Adult Rat Heart, Their Origin and Regulation by Es. American Society of Anesthesiology. A-536

66. Eghbali M., Deva R., Alioua A., Toro L., & Stefani E., (2005). Molecular and Functional Signature of Heart Hypertrophy during Pregnancy American Society of Anesthesiology. A-505

67. Alioua A., Lu R., Kumar Y., Eghbali M. & Toro L. (2005). Molecular Determinants of the Large-Conductance Voltage- and Ca 2+ -Activated K + Channel and Caveolin-1 Direct Physical Interac American Society of Anesthesiology. A-511

68. Eghbali M., Foroughi S., Kundu P., Toro L.& Stefani E. (2005).  Regulation of Heart MiRP1 Gene Activity by Estrogen. American Society of Anesthesiology. A431

69. Vallejo J., Eghbali M., Toro L.& Stefani E. (2006).  Altered Trafficking of Large Conductance Ca2+-activated K+ (MaxiK) Channels in Mouse Myometrium During Late Pregnancy. Biophysical Journal, 452-P.

70. Eghbali M., Kundu P., Foroughi S., Toro L.& Stefani E. (2006).  Estrogen Regulates the Transcriptional Activity of Cardiac MiRP1 Gene. Biophysical Journal, 2273-Platform.

71. Gomes P., Corsso C.D., Satio T., Eghbali M., Toro L.& Stefani E. (2006). Modulation of Kv4.3 channels by c-Src tyrosine kinase dependent phosphorylation. Biophysical Journal, 2584-P.

72. Minosyan T.Y. , Lu R., Eghbali M., Toro L., & Stefani, E. (2006). 5HT2A receptor upregulation in late pregnancy correlates with increased 5Htinduced Contractility. American Society of Anesthesiology.A-12.

73. Vallejo J., Eghbali M., Toro L.& Stefani E. (2006). Administration of Estrogen Induces the Upregulation of MaxiK Channels in Mouse Myometrium. American Society of Anesthesiology. A-13.

74. Tang G., Eghbali M., Minosyan T.Y. , Toro L., & Stefani, E. (2006). Modulation of cardiac Ito current and Kv4.3 channels by Src tyrosine kinase. American Society of Anesthesiology. A-379.

75. Saito T., Tang G., Eghbali M., Toro L., & Stefani, E. (2006). Gender Rules Transient Outward Currents in Mouse Right Ventricular Myocytes. American Society of Anesthesiology.A-649.

76. Fishfader P., Kirkpatrick V., Eghbali M., Minosyan T.Y. &Stefani E. (2006). Estrogen Receptor alpha, Caveolin and alpha-Actinin Form a Macromolecular Complex in the Heart. American Society of Anesthesiology. A-659

77. Ciobotaru A., Eghbali M., Kundu P., Toro L.& Stefani E. (2006). Estrogen Upregulates MiRP1 Gene Transcript Levels Via a Genomic Mechanism. American Society of Anesthesiology. A-660.

78. Saito T., Eghbali M., Vallejo J.,Toro L. & Stefani, E. (2007). Gender Regulates the Transient Outward K+ Currents in Mouse Right Ventricular Myocytes. Biophysical Journal, 2212-pos.

79. Fishfader P., Minosyan T.Y.,Eghbali M.,. Toro L. &Stefani E. (2007). Distinct Regulation of Estrogen Receptor Alpha Isoforms by Estrogen. Biophysical Journal,2957-Pos.

80. Minosyan T.Y., Lu R., Eghbali M., Toro L. & Stefani, E. (2007). Increased 5-HT Contractile Response in Late Pregnant Rat Myometrium May Result from a Higher Density of 5-HT2A Receptors. Biophysical Journal ,611-Pos.

81. Minosyan T.Y., Ciobotaru A., Toro L., Stefani, E. & Eghbali M. (2007). Caveolin 3 Associates with KCNE2 and ERG1 in Adult Mouse Heart. Biophysical Journal, 584-Pos.

82. Eghbali M., Ren S., Wang Y., Toro L. & Stefani E. Remodeling of KCNE2 Transcripts and Protein Subcellular Localization in Decompensated Heart Failure. Submitted to American Society of Anesthesiology. A-1025

83. Minosyan T.Y., Saito T.,Toro L., Stefani, E. & Eghbali M. KCNE2 Associates with Caveolin 3 and ERG1 in Adult Mouse Heart. Submitted to American Society of Anesthesiology. A-622

84. Ciobotaru A., Kundu P., Toro L., Stefani E. & Eghbali M. Estrogen Stimulation of KCNE2 (MiRP1) Promoter Activity by a Direct Genomic Mechanism. Submitted to American Society of Anesthesiology. A-1023

85. Saito T., Minosyan T.Y., Eghbali M., Toro L. & Stefani, E. Heart Pre-crosslinking Prior to Cell Dissociation Stabilizes Fine Cardiomyocyte Cytoarchitecture. Submitted to American Society of Anesthesiology. A-614